Effect of sucrose pre-culture on cryopreservation by vitrification of Sideritis leucantha Cav. subsp. leucantha seedlings produced in vitro
Abstract
Sideritis leucantha Cav. subsp. leucantha is a Valencian endemic medicinal plant that faces some conservation constraints. Therefore, this work aimed to test a preconditioning treatment for the further cryopreservation of shoot tip explants of this species. The results showed that pre-culture in sucrose 0.3 M for 24 hours resulted in the highest explant survival and regeneration after three vitrification approaches. These findings point out that preconditioning of explants may enhance cryopreservation performance in tissue cultured plantlets of S. leucantha subps. leucantha.
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