Nereis. Interdisciplinary Ibero-American Journal of Methods, Modelling and Simulation.


Effect of sucrose pre-culture on cryopreservation by vitrification of Sideritis leucantha Cav. subsp. leucantha seedlings produced in vitro


Sideritis leucantha Cav. subsp. leucantha is a Valencian endemic medicinal plant that faces some conservation constraints. Therefore, this work aimed to test a preconditioning treatment for the further cryopreservation of shoot tip explants of this species. The results showed that pre-culture in sucrose 0.3 M for 24 hours resulted in the highest explant survival and regeneration after three vitrification approaches. These findings point out that preconditioning of explants may enhance cryopreservation performance in tissue cultured plantlets of S. leucantha subps. leucantha.


Barber JC, Santos-Guerra A, Turner KG, Jansen R.K. Origin of Macaronesian Sideritis L. (Lamioideae: Lamiaceae) inferred from nuclear and chloroplast sequence datasets, Mol. Phylogenet. Evol. 2002;23(3):293-306.

Font Quer P. Plantas Medicinales. Ed. Labor, Barcelona, Spain; 1962.

González-Burgos E, Carretero ME, Gómez-Serranillos MP. Sideritis spp.: Uses, chemical composition and pharmacological activities—A review. J Ethnopharmacol. 2011;35:209–225.

Pardo de Santayana M, Morales R, Tardío J, Molina M. Inventario español de los conocimientos tradicionales relativos a la biodiversidad. Fase II. Ministerio de Agricultura y Pesca, Alimentación y Medio Ambiente. Madrid; 2018.

Barberán FAT, Tomás F, Ferreres. Two flavone glycosides from Sideritis leucantha. Phytochemistry. 1984;9:2112–2113

Barberan FAT, Mañez S, Villar. Identification of antiinflammatory agents from Sideritis species growing in Spain. J Nat Prod. 1987;50: 313–314

Iscan G, Kirimer N, Kurkcuoglu M, Baser KHC. Composition and antimicrobial activity of the essential oils of two endemic species from Turkey: Sideritis cilicica and Sideritis bilgerana. Chem Nat Comp. 2005;41:679–682.

Dulger B, Gonuz A, Aysel V. Inhibition of clotrimazole-resistant Candida albicans by some endemic Sideritis species from Turkey. Fitoterapia. 2006;77:404–405

Tsaknis J, Lalas S. Extraction and identification of natural antioxidant from Sideritis euboea (mountain tea). J Agric Food Chem. 2005;53:6375–6381

Laguna E. Flora rara, endémica o amenazada de la Comunidad Valenciana. Generalitat Valenciana, Conselleria de Medio Ambiente, València ; 1998.

Allen DJ. 2014. Sideritis leucantha. The IUCN Red List of Threatened Species 2014: e.T203269A2762702.

Reed BM. Plant Cryopreservation: A Practical Guide. Springer-Verlag New York; 2008.

González-Benito ME, Martín C. In vitro preservation of Spanish biodiversity. In Vitro Cell Dev Biol Plant. 2011;47:46–54

Charoensub R, Phansiria S, Yongmanitchai W, Sakai A. Routine cryopreservation of in vitro-grown axillary apices of cassava (Manihot esculenta Crantz) by vitrification: importance of a simple mononodal culture. Sci Horticult. 2003;98:485–492

Towill LE. Cryopreservation by vitrification. In: B. Grout (ed) Genetic Preservation of plant cells in vitro. Springer Verlag New York; 1995.

Channuntapipat C, Collins G, Sedgley M. Conservation of almond germplasm by cryopreservation. Cahiers Options Méditerranéennes. 2001;56:101-106

Takagi H, Tien Thinh N, Islam OM, Senboku T, Sakai A. Cryopreservation of in vitro.grown shoot tips of taro (Colocasia esculenta (L.) Schott) by vitrification. 1. Investigation of basic conditions of the vitrification procedure. Plant Cell Rep. 1997;16:594-599

Panis B, Lambardi M. Status of cryopreservation technologies in plants (crops and forest trees). The role of biotechnology. Villa Gualino, Turin, Italy – 5-7 March; 2005

Engelmann F. Plant cryopreservation: Progress and prospects. In vitro Cell.& Dev. Biol. Plant. 2004;40:427-433.

Hirai D, Sakai A. Cryopreservation of in vitro-grown axillary shoot-tip meristems of mint (Mentha spicata L.) by encapsulation vitrification. Plant Cell Rep. 1998;19:150–155

Luo J and Reed BM. Abscisic Acid-Responsive Protein, Bovine Serum Albumin, and Proline Pretreatments Improve Recovery of in Vitro Currant Shoot-Tip Meristems and Callus Cryopreserved by Vitrification. CryoBiology. 1997;34:240–250

Efendi D. Transformation and cryopreservation of embryogenic avocado (Persea americana Mill.) cultures. Dissertation for the degree of Doctor of Philosophy. University of Florida; 2003.

Lambardi M, Fabbri A, Caccavale A. Cryopreservation of white poplar (Populus alba L.) by vitrification of in vitro-grown shoot tips. Plant Cell Rep. 2000;19:213–218

Volk GM, Walters C. Plant vitrification solution 2 lowers water content and alters freezing behavior in shoot tips during cryoprotection. Cryobiology. 2006;52(1):48–61

Hirai D, Sakai A. Simplified cryopreservation of sweet potato [Ipomoea batatas (L.) Lam.] by optimizing conditions for osmoprotection. Plant Cell Rep. 2003;21:961–966

Matsumoto T, Sakai A. Cryopreservation of axillary shoot tips of in vitro-grown grape (Vitis) by a two-step vitrification protocol. Euphytica. 2003; 131:299–304

Halmagyi A, Pinker I. Plant regeneration from Rosa shoot tips cryopreserved by a combined droplet vitrification method. Plant Cell Tissue Organ Cult. 2006;84:145–153

Pennycooke JC, Towill LE. Cryopreservation of shoot tips from in vitro plants of sweet potato [Ipomoea batatas (L.) Lam.] by vitrification. Plant Cell Rep. 2000;19:733–737

Langis R., Schneibel B, Earle ED, Steponkus PL. Cryopreservation of carnation shoot tips by vitrification. Cryobiology. 1990;27:657–658

Benson EE. Cryopreservation. Plant cell culture. A practical approach. 2nd ed. Oxford University Press, London; 1996.

Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Plant Physiol. 1962;15:473–497

Ahuja S, Mandal BB, Dixit S, Srivastava PS. Molecular, phenotypic and biosynthetic stability in Dioscorea floribunda plants derived from cryopreserved shoot tips. Plant Sci. 2002;163:971-977

Matsumoto T, Takahashi C, Sakai A, Nako Y. Cryopreservation of in vitro-grown apical meristemos of hybrid statice by three different procedures. Scientia Horticult. 1998;76:105–114

Tannoury M, Ralabosoa J, Kaminski M, Dereuddre. Cryoconservation par vitrification d’apex enrobés d’œillet (Dianthus caryophyllus L.) cultivé in vitro.C. R. Acad. Sci. Paris. 1991;313:633–638

Tannoury M, Vintéjoux C, Dereuddre. Cryoconservation par encapsulation et déhydratation d’apex d’œillet (Dianthus caryophyllus L.) cultivés in vitro.C. Acta bot. Gallica. 1995;142(5):415–424

Volk GM, Caspersen AM. Plasmolysis and recovery of different cell types in cryoprotected shoot tips of Mentha x piperita. Protoplasma. 2007;231:215–226


Download data is not yet available.