Effect of water activity and temperature on the growth and sporulation of Aspergillus niger P.E.L. van Tieghem
The effect of water activity (0.85, 0.90, 0.95, 0.98, 0.995) on the growth and sporulation of Aspergillus niger was studied at different temperatures (15 and 25ºC). Maximum fungal growth rate occurred at water activity of 0.98, with growth being observed throughout the tested interval except at 0.85 and 15ºC. In addition, morphological and cultural features were studied in the different experimental conditions. This species sporulated at almost all conditions. Both factors and their interaction had a significant effect on the development of the species.
De Vries RP, Riley R, Wiebenga A, Aguilar-Osorio G, Amillis S, Uchima CA, et al. Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus. Genome Biol 2017;18.
Behera BC. Citric acid from Aspergillus niger: a comprehensive overview. Critical Reviews in Microbiology 2020; 46:727–49.
Li C, Zhou J, Du G, Chen J, Takahashi S, Liu S. Developing Aspergillus niger as a cell factory for food enzyme production. Biotechnology Advances 2020; 44:107630.
Differences in metabolites production using the Biolog FF MicroplateTM system with an emphasis on some organic acids of Aspergillus niger wild type strains,Biologia - X-MOL n.d. https://www.x-mol.com/paper/1263906105200844800 (accessed January 23, 2021).
Perrone G, Gallo A. Aspergillus species and their associated mycotoxins. In: Moretti A, Susca A, editors. Mycotoxigenic fungi: methods and protocols, New York, NY: Springer; 2017, p. 33–49.
Sempere F, Santamarina MP. In vitro biocontrol analysis of Alternaria alternata (Fr.) Keissler under different environmental conditions. Mycopathologia 2007; 163:183–90.
García de Lomas J, Marín F, Altuna A, Cuadrado E. A simple and rapid method for microculture and identification of fungi. Mycopathologia 1981; 76:119–24.
Parra R, Aldred D, Archer DB, Magan N. Water activity, solute and temperature modify growth and spore production of wild type and genetically engineered Aspergillus niger strains. Enzyme and Microbial Technology 2004; 35:232–7.
Astoreca A, Magnoli C, Ramirez ML, Combina M, Dalcero A. Water activity and temperature effects on growth of Aspergillus niger, A. awamori and A. carbonarius isolated from different substrates in Argentina. Int J Food Microbiol 2007; 119:314–8.
Leong SL, Hocking AD, Scott ES. Effect of temperature and water activity on growth and ochratoxin A production by Australian Aspergillus carbonarius and A. niger isolates on a simulated grape juice medium. Int J Food Microbiol 2006; 110:209–16.
Gerez CL, Dallagnol A, Ponsone L, Chulze S, Font de Valdez G. Ochratoxin A production by Aspergillus niger: Effect of water activity and a biopreserver formulated with Lactobacillus plantarum CRL 778. Food Control 2014; 45:115–9.
Lasram S, Hamdi Z, Chenenaoui S, Mliki A, Ghorbel A. Comparative study of toxigenic potential of Aspergillus flavus and Aspergillus niger isolated from Barley as affected by temperature, water activity and carbon source. Journal of Stored Products Research 2016; 69:58–64.
Alborch L, Bragulat MR, Abarca ML, Cabañes FJ. Effect of water activity, temperature and incubation time on growth and ochratoxin A production by Aspergillus niger and Aspergillus carbonarius on maize kernels. Int J Food Microbiol 2011; 147:53–7.
Marin S, Sanchis V, Ramos AJ, Vinas I, Magan N. Environmental factors, in vitro interactions, and niche overlap between Fusarium moniliforme, F. proliferatum, and F. graminearum, Aspergillus and Penicillium species from maize grain. Mycological Research 1998; 102:831–7.
Esteban A, Abarca ML, Bragulat MR, Cabañes FJ. Effect of water activity on ochratoxin A production by Aspergillus niger aggregate species. Int J Food Microbiol 2006; 108:188–95.
Parra R, Magan N. Modelling the effect of temperature and water activity on growth of Aspergillus niger strains and applications for food spoilage moulds. J Appl Microbiol 2004; 97:429–38.
Palacios-Cabrera H, Taniwaki MH, Hashimoto JM, Menezes HC de. Growth of Aspergillus ochraceus, A. carbonarius and A. niger on culture media at different water activities and temperatures. Brazilian Journal of Microbiology 2005; 36:24–8.