Effect of sucrose pre-culture on cryopreservation by vitrification of Sideritis leucantha Cav. subsp. leucantha seedlings produced in vitro


  • Jorge Instituto de Investigación en Medio Ambiente y Ciencia Marina (IMEDMAR). Universidad Católica de Valencia San Vicente Mártir.
  • Jorge Eliseo Ramírez-Luna Instituto Universitario de Investigación CIBIO (Centro Iberoamericano de la Biodiversidad), Universidad de Alicante.
  • Abel Piqueras Departamento de Mejora Vegetal. Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC).
  • José Luis Casas Instituto Universitario de Investigación CIBIO (Centro Iberoamericano de la Biodiversidad), Universidad de Alicante.




Cryopreservation, endemic plants, in vitro culture, medicinal plants, preconditioning


Sideritis leucantha Cav. subsp. leucantha is a Valencian endemic medicinal plant that faces some conservation constraints. Therefore, this work aimed to test a preconditioning treatment for the further cryopreservation of shoot tip explants of this species. The results showed that pre-culture in sucrose 0.3 M for 24 hours resulted in the highest explant survival and regeneration after three vitrification approaches. These findings point out that preconditioning of explants may enhance cryopreservation performance in tissue cultured plantlets of S. leucantha subps. leucantha.


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