Essential Oils as Natural Antimicrobial and Antioxidant Products in the Agrifood Indus

Authors

  • M. Dolores Ibáñez Departament de Farmacologia. Facultat de Farmàcia. Universitat de València.
  • M. Pilar López-Gresa Instituto de Biología Molecular y Celular de Plantas. Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas.
  • Purificación Lisón Instituto de Biología Molecular y Celular de Plantas. Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas.
  • Ismael Rodrigo Instituto de Biología Molecular y Celular de Plantas. Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas.
  • José María Bellés Instituto de Biología Molecular y Celular de Plantas. Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas.
  • M. Carmen González-Mas Departament de Farmacologia. Facultat de Farmàcia. Universitat de València.
  • M. Amparo Blázquez Departament de Farmacologia. Facultat de Farmàcia. Universitat de València.

DOI:

https://doi.org/10.46583/nereis_2020.12.585

Keywords:

essential oils, agrifood industry, antioxidant activity, antibacterial activity, antifungal activity

Abstract

Consumers are aware of the dangers arising from the use of synthetic antioxidants and antimicrobials in the agrifood industry, demanding safer and “greener” alternatives. In this study, the antioxidant activity of commercial essential oils through DPPH method, their antimicrobial effects against the bacterium Pseudomonas syringae and the phytopathogenic fungus Fusarium oxysporum by means of the standardized disk method were determined. Clove along with winter savory, cinnamon and oregano essential oils as well as carvacrol showed the highest antioxidant activity comparable to reference standards. Wintergreen essential oil was the most potent inhibitor against P. syringae growth at the highest doses (20 and 10 ?L). Oregano essential oil and its main component carvacrol were able to stop the bacterium growth even at the lowest treatment (1 ?L). Cinnamon, oregano and peppermint essential oils inhibited F. oxysporum development at all doses (20, 10 and 5 ?L) assayed. In general, most of the essential oils displayed more antifungal than antibacterial and antioxidant activities.

References

Prakash B, Kedia A, Mishra PK, Dubey NK. Plant essential oils as food preservatives to control moulds, mycotoxin contamination and oxidative deterioration of agri-food commodities-Potentials and challenges. Food Control. 2015;47(1):381-91.

Rodriguez-Garcia I, Silva-Espinoza BA, Ortega-Ramirez LA, Leyva JM, Siddiqui MW, Cruz-Valenzuela MR et al. Oregano essential oil as an antimicrobial and antioxidant additive in food products. Crit Rev Food Sci Nutr. 2016;56(10):1717-27.

Rodriguez-Garcia I, Cruz-Valenzuela MR, Silva-Espinoza BA, Gonzalez-Aguilar GA, Moctezuma E, Gutierrez-Pacheco MM et al. Oregano (Lippia graveolens) essential oil added within pectin edible coatings prevents fungal decay and increases the antioxidant capacity of treated tomatoes. J Sci Food Agric. 2016;96(11):3772-8.

Mohammadi A, Hashemi M, Hosseini SM. Postharvest treatment of nanochitosan-based coating loaded with Zataria multiflora essential oil improves antioxidant activity and extends shelf-life of cucumber. Innov Food Sci Emerg Technol. 2016;33:580-8.

Brewer MS. Natural antioxidants: sources, compounds, mechanisms of action, and potential applications. Compr Rev Food Sci Food Saf. 2011;10(4):221-47.

Mendes R de F, Pinto N de CC, da Silva JM, da Silva JB, Hermisdorf RC do. S, Fabri RL, et al. The essential oil from the fruits of the Brazilian spice Xylopia sericea A. St.-Hil. presents expressive in-vitro antibacterial and antioxidant activity. J Pharm Pharmacol. 2017;69(3):341-8.

Semdé Z, Koudou J, Figueredo G, Zongo C, Somda KM, Sawadogo/Lingani H, et al. Chemical composition, antioxidant and antimicrobial activities of Lantana camara Linn leaves essential oil from Burkina Faso. GSC Biol Pharm Sci. 2018;5(3):124-35.

Isman M, Miresmailli S, Machial C. Commercial opportunities for pesticides based on plant essential oils in agriculture, industry and consumer products. Phytochem Rev. 2011;10(2):197-204.

Tyagi AK, Malik A. Antimicrobial potential and chemical composition of Eucalyptus globulus oil in liquid and vapour phase against food spoilage microorganisms. Food Chem. 2011;126(1):228-35.

Tyagi AK, Malik A. Antimicrobial potential and chemical composition of Mentha piperita oil in liquid and vapour phase against food spoiling microorganisms. Food Control. 2011;22(11):1707-14.

Boukhatem MN, Kameli A, Saidi F. Essential oil of Algerian rose-scented geranium (Pelargonium graveolens): Chemical composition and antimicrobial activity against food spoilage pathogens. Food Control. 2013;34(1):208-13.

Amini M, Safaie N, Salmani MJ, Shams-Bakhsh M. Antifungal activity of three medicinal plant essential oils against some phytopathogenic fungi. Trakia J Sci. 2012;10(1):1-8.

Badawy MEI, Abdelgaleil SAM. Composition and antimicrobial activity of essential oils isolated from Egyptian plants against plant pathogenic bacteria and fungi. Ind Crops Prod. 2014;52:776-82.

Almeida ET da C, de Souza GT, de Sousa Guedes JP, Barbosa IM, de Sousa CP, Castellano LRC et al. Mentha piperita L. essential oil inactivates spoilage yeasts in fruit juices through the perturbation of different physiological functions in yeast cells. Food Microbiol. 2019;82:20-9.

Shi Y, Huang S, He Y, Wu J, Yang Y. Navel orange peel essential oil to control food spoilage molds in potato slices. J Food Prot. 2018;81(9):1496-502.

Plazas M, López-Gresa MP, Vilanova S, Torres C, Hurtado M, Gramazio P, Andújar I, Herráiz FJ, Bellés JM, Prohens J. Diversity and relationships in key traits for functional and apparent quality in a collection of eggplant: fruit phenolics content, antioxidant activity, polyphenol oxidase activity, and browning. J Agric Food Chem. 2013; 61(37):8871-9.

López-Gresa MP, Payá C, Ozáez M, Rodrigo I, Conejero V, Klee H, Bellés JM, Lisón P. A new role for green leaf volatile esters in tomato stomatal defense against Pseudomonas syringe pv. tomato. Front Plant Sci. 2018;18(9):1855.

Hernández Aparicio FJ. Estudio de la respuesta defensiva de plantas de tomate frente a una infección virulenta o avirulenta causada por Fusarium oxysporum f. sp. lycopersici. Trabajo Final de Máster. Universitat Politècnica de València. 2018.

Amorati R, Foti MC, Valgimigli L. Antioxidant activity of essential oils. J Agric Food Chem. 2013;61(46):10835-47.

Xu D, Hu MJ, Wang YQ, Cui YL. Antioxidant activities of Quercetin and its complexes for medicinal applications. Molecules. 2019;24:1123.

Anwar H, Hussain G, Mustafa I. Antioxidants from natural sources. In: Shalaby E, Azzam GM, editors. Antioxidants in foods and its applications. London: IntechOpen; 2018. p. 2-28.

Zhang M, Swarts SG, Yin L, Liu C, Tian Y, Cao Y, et al. Antioxidant properties of Quercetin Adv Exp Med Biol. 2011;701:283-9.

Viuda-Martos M, Ruiz Navajas Y, Sánchez Zapata E, Fernández-López J, Pérez-Álvarez JA. Antioxidant activity of essential oils of five spice plants widely used in a Mediterranean diet. Flavour Fragr J. 2009;25(1):13-9.

Saricaoglu FT, Turhan S. Antimicrobial activity and antioxidant capacity of thyme, rosemary and clove essential oils and their mixtures. J Innov Sci Eng. 2018;2(1):25-33.

Gülçin I, Elmastaş M, Aboul-Enein HY. Antioxidant activity of clove oil-A powerful antioxidant source. Arab J Chem. 2012;5(4):489-99.

Kumar D, Mehta N, Chatli MK, Kaur G, Malav OP, Kumar P. In-vitro assessment of antimicrobial and antioxidant potential of essential oils from Lemongrass (Cymbopogon citratus), Cinnamon (Cinnamomum verum) and Clove (Syzygium aromaticum). J Anim Res. 2017;7(6):1099-105.

Ugalde ML, de Cezaro AM, Vedovatto F, Paroul N, Steffens J, Valduga E, et al. Active starch biopolymeric packaging film for sausages embedded with essential oil of Syzygium aromaticum. J Food Sci Technol. 2017;54(7):2171-5.

Özcan MM, Arslan D. Antioxidant effect of essential oils of rosemary, clove and cinnamon on hazelnut and poppy oils. Food Chem. 2011;129(1):171-4.

Coutinho de Oliveira TL, Malfitano de Carvalho S, de Araújo Soares R, Andrade MA, Cardoso M das G, Ramos EM, et al. Antioxidant effects of Satureja montana L. essential oil on TBARS and color of mortadella-type sausages formulated with different levels of sodium nitrite. LWT - Food Sci Technol. 2012;45(2):204-12.

Šojić B, Pavlić B, Tomović V, Ikonić P, Zeković Z, Kocić-Tanackov S, et al. Essential oil versus supercritical fluid extracts of winter savory (Satureja montana L.)–Assessment of the oxidative, microbiological and sensory quality of fresh pork sausages. Food Chem. 2019;287:280-6.

El-Baroty GS, Abd El-Baky HH, Farag RS, Saleh MA. Characterization of antioxidant and antimicrobial compounds of cinnamon and ginger essential oils. African J Biochem Res. 2010;4(6):167-74.

Echegoyen Y, Nerín C. Performance of an active paper based on cinnamon essential oil in mushrooms quality. Food Chem. 2015;170:30-6.

Black-Solis J, Ventura-Aguilar RI, Correa-Pacheco Z, Corona-Rangel ML, Bautista-Baños S. Preharvest use of biodegradable polyester nets added with cinnamon essential oil and the effec on the storage life of tomatoes and the development of Alternaria alternata. Sci Hortic (Amsterdam). 2019;245:65-73.

Fasihi H, Noshirvani N, Hashemi M, Fazilati M, Salavati H, Coma V. Antioxidant and antimicrobial properties of carbohydrate-based films enriched with cinnamon essential oil by Pickering emulsion method. Food Packag Shelf Life. 2019;19:147-54.

Gavaric N, Mozina SS, Kladar N, Bozin B. Chemical profile, antioxidant and antibacterial activity of thyme and oregano essential oils, thymol and carvacrol and their possible synergism. J Essent Oil-Bearing Plants. 2015;18(4):1013-21.

Olmedo R, Ribotta P, Grosso NR. Decrease of chemical and volatile oxidation indicators using oregano essential oil combined with BHT in sunflower oil under accelerated storage conditions. J Food Sci Technol. 2019;56(5):2522-35.

Rodrigues Fernandes Ferreira V, Lee Nelson D, Saczk AA, da Silva Felix F, Magalhaex Brandao R, Cardoso M das G. Chelating effect of carvacrol and the oregano essential oil. In: 49th International Symposium on Essential Oils(ISEO2018). 2018:99.

Neira LM, Martucci JF, Stejskal N, Ruseckaite RA. Time-dependent evolution of properties of fish gelatin edible films enriched with carvacrol during storage. Food Hydrocoll. 2019;94(12):304-10.

Fonseca LM, Cruxen CE dos S, Bruni GP, Fiorentini ÂM, Zavareze E da R, Lim L-T et al. Development of antimicrobial and antioxidant electrospun soluble potato starch nanofibers loaded with carvacrol. Int J Biol Macromol. 2019;139:9.

Wieczyńska J, Cavoski I. Antimicrobial, antioxidant and sensory features of eugenol, carvacrol and trans-anethole in active packaging for organic ready-to-eat iceberg lettuce. Food Chem. 2018;259:251-60.

Rúa J, del Valle P, de Arriaga D, Fernández-Álvarez L, García-Armesto MR. Combination of carvacrol and thymol: antimicrobial activity against Staphylococcus aureus and antioxidant activity. Foodborne Pathog Dis. 2019;16(9):622-9.

Ibáñez MD, Blázquez MA. Essential oil quality of green tea (Camellia sinensis (L.) Kuntze) in commercial samples. Int J Pharmacogn Phytochem. 2012;31(1):1380-4.

Hendel N, Napoli E, Sarri M, Saija A, Cristani M, Nostro A et al. Essential oil from aerial parts of wild algerian rosemary: screening of chemical composition, antimicrobial and antioxidant activities. J Essent Oil-Bearing Plants. 2019;22(1):1-17.

Kanth MK, Mehta N, Kumar CM, Prakash MO, Kumar P, Wagh RV et al. In-vitro assessment of antimicrobial, antibiofilm and antioxidant potential of essential oil from rosemary (Rosmarinus officinalis L.). J Anim Res. 2018;8(6):989-98.

Poaty B, Lahlah J, Porqueres F, Bouafif H. Composition, antimicrobial and antioxidant activities of seven essential oils from the North American boreal forest. World J Microbiol Biotechnol. 2015;31(6):907-19.

Sing HP, Kaur S, Negi K, Kumari S, Saini V, Batish DR, et al. Assessment of in vitro antioxidant activity of essential oil of Eucalyptus citriodora (lemon-scented Eucalypt; Myrtaceae) and its major constituents. LWT-Food Sci Technol. 2012;48:237-24.

Harkat-Madouri L, Asma B, Madani K, Bey-Ould Si Said Z, Rigou P, Grenier D, et al. Chemical composition, antibacterial and antioxidant activities of essential oil of Eucalyptus globulus from Algeria. Ind Crops Prod. 2015;78:148-53.

Nikolić M, Marković T, Mojović M, Pejin B, Savić A, Perić T et al. Chemical composition and biological activity of Gaultheria procumbens L. essential oil. Ind Crops Prod. 2013;49:561-7.

Brado Avanço G, Dias Ferreira F, Silva Bomfim N, de Souza Rodrigues dos Santos PA, Peralta RM, Brugnari T, et al. Curcuma longa L. essential oil composition, antioxidant effect, and effect on Fusarium verticillioides and fumonisin production. Food Control. 2017;73:806-13.

Uppalapati SR, Ishiga Y, Wangdi T, Urbanczyk-Wochniak E, Ishiga T, Mysore KS et al. Pathogenicity of Pseudomonas syringae pv. tomato on tomato seedlings: Phenotypic and gene expression analyses of the virulence function of coronatine. Mol Plant-Microbe Interact. 2008;21(4):383-95.

González I, Arias Y, Peteira B. Aspectos generales de la interacción Fusarium oxysporum f. sp. lycopersici-tomate. Rev Protección Veg. 2012;27(1):1-7.

Alkan D, Yemenicioğlu A. Potential application of natural phenolic antimicrobials and edible film technology against bacterial plant pathogens. Food Hydrocoll. 2016;55:1-10.

Nostro A, Papalia T. Antimicrobial activity of carvacrol: Current progress and future prospectives. Recent Pat Antiinfect Drug Discov. 2012;7(1):28-35(8).

Oliva MM, Carezzano ME, Giuliano M, Daghero J, Zygadlo J, Bogino P et al. Antimicrobial activity of essential oils of Thymus vulgaris and Origanum vulgare on phytopathogenic strains isolated from soybean. Plant Biol. 2015;17(3):758-65.

Božik M, Nový P, Klouček P. Chemical composition & antimicrobial activity of cinnamon, thyme, oregano & clove essential oils against plant pathogenic bacteria. Acta Univ Agric Silvic Mendelianae Brun. 2017;65(4):1129-34.

Bravo Cadena M, Preston GM, Van der Hoorn RAL, Townley HE, Thompson IP. Species-specific antimicrobial activity of essential oils and enhancement by encapsulation in mesoporous silica nanoparticles. Ind Crops Prod. 2018;122:582-90.

Carson CF, Hammer KA, Riley TV. Melaleuca alternifolia (tea tree) oil: A review of antimicrobial and other medicinal roperties. Clin Microbiol Rev. 2006;19(1):50-62.

Sabir A, El-Khalfi B, Errachidi F, Chemsi I, Serrano A, Soukri A. Evaluation of the potential of some essential oils in biological control against phytopathogenic agent Pseudomonas syringae pv. tomato DC3000 responsible for the tomatoes speck. J Plant Pathol Microbiol. 2017;8(9):420.

Oliveira da Silva É, Martins SJ, Alves E. Essential oils for the control of bacterial speck in tomato crop. African J Agric Res. 2014;9(34):2624-9.

Božik M, P. Nový P, Klouček P. Susceptibility of postharvest pathogens to essential oils. Scientia Agriculturae Bohemica 2017;48(3):103-11.

Diánez F, Santos M, Parra C, Navarro MJ, Blanco R, Gea FJ. Screening of antifungal activity of 12 essential oils against eight pathogenic fungi of vegetables and mushroom. Lett Appl Microbiol. 2018;67(4):400-10.

Park JY, Jim SH, Kim NH, Lee SW, Jeun YC, Hong JK. Differential inhibitory activities of four plant essential oils on in vitro growth of Fusarium oxysporum f. sp. fragariae causing fusarium wilt in strawberry plants. Plant Pathol J. 2017;33(6):582-8.

Riccioni L, Orzali L. Activity of tea tree (Melaleuca alternifolia, Cheel) and thyme (Thymus vulgaris, Linnaeus.) essential oils against some pathogenic seed borne fungi. J Essent Oil Res. 2011;23(6):43-7.

Kong Q, Zhang L, An P, Qi J, Yu X, Lu J, et al. Antifungal mechanisms of α-terpineol and terpene-4-alcohol as the critical components of Melaleuca alternifolia oil in the inhibition of rot disease caused by Aspergillus ochraceus in postharvest grapes. J Appl Microbiol. 2019;126(4):1161-74.

Hussein KA, Joo JH. Antifungal activity and chemical composition of ginger essential oil against ginseng pathogenic fungi. Curr Res Environ Appl Mycol. 2018;8(2):194-203.

Santamaria MP, Ibáñez MD, Marqués M, Roselló J, Gímenez S, Blázquez MA. Bioactivity of essential oils in phytopathogenic and post-harvest fungi control. Nat Prod Res. 2017;32(22):2675-9.

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Published

2020-07-16