Nereis. Interdisciplinary Ibero-American Journal of Methods, Modelling and Simulation.

Buscador

Inhibition of motility and Quorum sensing bacteria due to winemaking waste

Abstract

In the food industry, biofilm is the main cause of contamination. Due to food regulations, only a few compounds can be used as preservatives or be in contact with food during their preparation and, at the same time, they must be economical. An alternative is the use of by-products or agro-industry waste. One of the main food industries in Latin America is viticulture. The present research uses marc and stems of Torrontés varietal wine to inhibit the bacterial virulence controlled by Quorum sensing (QS) and its motility (swarming and swimming), which would allow the displacement and formation of biofilm in foods. We perform extractions with solvents of increasing polarity (hexane, chloroform, ethyl acetate, and methanol). We compare 250 and 500 ?g / ml of extracts to the strains of Pseudomonas aeruginosa ATCC 27853 and LVP65. Methanolic extracts to marc and stems inhibited more than 50 swarming motility of both strains. In addition, both ethyl acetate extracts inhibited the swimming (25-30 %). The anti-QS activity of the Extracts was tested with strains Chromobacterium violaceum ATCC 12472 and CV026 and an inhibition of the methanolic and acetate extracts on the QS system was determined. The bioactivities found make a novel alternative for food preservation out of this waste.

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