Inhibition of motility and Quorum sensing bacteria due to winemaking waste

Authors

  • Carolina María Viola Instituto de Biotecnología Farmacéutica y alimentaria (INBIOFAL). CONICET- Universidad Nacional de Tucumán.
  • María Rosa Alberto Instituto de Biotecnología Farmacéutica y alimentaria (INBIOFAL). CONICET- Universidad Nacional de Tucumán./Facultad de Bioquímica, Química y Farmacia. Universidad Nacional de Tucumán.
  • Elena Cartagena Instituto de Biotecnología Farmacéutica y alimentaria (INBIOFAL). CONICET- Universidad Nacional de Tucumán. / Facultad de Bioquímica, Química y Farmacia. Universidad Nacional de Tucumán.
  • Mario Eduardo Arena Instituto de Biotecnología Farmacéutica y alimentaria (INBIOFAL). CONICET- Universidad Nacional de Tucumán. / Facultad de Bioquímica, Química y Farmacia. Universidad Nacional de Tucumán.

DOI:

https://doi.org/10.46583/nereis_2020.12.580

Keywords:

agro-industrial waste, Quorum sensing, motility

Abstract

In the food industry, biofilm is the main cause of contamination. Due to food regulations, only a few compounds can be used as preservatives or be in contact with food during their preparation and, at the same time, they must be economical. An alternative is the use of by-products or agro-industry waste. One of the main food industries in Latin America is viticulture. The present research uses marc and stems of Torrontés varietal wine to inhibit the bacterial virulence controlled by Quorum sensing (QS) and its motility (swarming and swimming), which would allow the displacement and formation of biofilm in foods. We perform extractions with solvents of increasing polarity (hexane, chloroform, ethyl acetate, and methanol). We compare 250 and 500 ?g / ml of extracts to the strains of Pseudomonas aeruginosa ATCC 27853 and LVP65. Methanolic extracts to marc and stems inhibited more than 50 swarming motility of both strains. In addition, both ethyl acetate extracts inhibited the swimming (25-30 %). The anti-QS activity of the Extracts was tested with strains Chromobacterium violaceum ATCC 12472 and CV026 and an inhibition of the methanolic and acetate extracts on the QS system was determined. The bioactivities found make a novel alternative for food preservation out of this waste.

References

Horn N, Bhunia AK. Food-associated stress primes foodborne pathogens for the gastrointestinal phase of infection. Frontiers in Microbiology. 2018;9:1962. Frontiers Media S. A.

Waters CM, Bassler BL. QUORUM SENSING: Cell-to-Cell Communication in Bacteria. Annu Rev Cell Dev Biol. 2005;21(1):319-46.

Eickhoff MJ, Bassler BL. SnapShot: Bacterial Quorum Sensing. Cell. Cell Press. 2018;174:1328-1328.e1.

Antonioli L, Blandizzi C, Pacher P, Guilliams M, Haskó G. Quorum sensing in the immune system. Nature Reviews Immunology. Nature Publishing Group. 2018;18:537-8.

Juhas M, Eberl L, Tummler B. Quorum sensing: the power of cooperation in the world of Pseudomonas. Environ Microbiol. 2005;7(4):459-71.

Pallaval Veera Bramhachari. Introduction to Quorum sensing Research in Diverse Microbial Systems. En: Implication of Quorum Sensing System in Biofilm Formation and Virulence. Springer Singapore. 2018:3-6.

Shrout JD, Chopp DL, Just CL, Hentzer M, Givskov M, Parsek MR. The impact of quorum sensing and swarming motility on Pseudomonas aeruginosa biofilm formation is nutritionally conditional. Mol Microbiol. 2006;62(5):1264-77.

Ha DG, Kuchma SL, O’Toole GA. Plate-based assay for swarming motility in Pseudomonas aeruginosa. Methods Mol Biol. 2014;1149:67-72.

Ha DG, Kuchma SL, O’Toole GA. Plate-Based assay for swimming motility in pseudomonas aeruginosa. Methods Mol Biol. 2014;1149:59-65.

Ha D, Biofilms GO-M, 2015 undefined. c-di-GMP and its Effects on Biofilm Formation and Dispersion: a Pseudomonas Aeruginosa Review. Microbial Biofilms. 2015:301-317.

Koh C-L, Sam C-K, Yin W-F, Ying Tan L, Krishnan T, Meng Chong Y, et al. Plant-Derived Natural Products as Sources of Anti-Quorum Sensing Compounds. Sensors. 2013:6217-28.

Durán N, Justo GZ, Durán M, Brocchi M, Cordi L, Tasic L, et al. Advances in Chromobacterium violaceum and properties of violacein-Its main secondary metabolite: A review. Biotechnol Adv. 2016;34(5):1030-45.

Teixeira A, Baenas N, Dominguez-Perles R, Barros A, Rosa E, Moreno DA, et al. Natural bioactive compounds from winery by-products as health promoters: a review. Int J Mol Sci [Internet]. 4 de septiembre de 2014;15(9):15638-78.

Christ KL, Burritt RL. Critical environmental concerns in wine production: An integrative review. Journal of Cleaner Production. 2013; 53:232-42.

Dwyer K, Hosseinian F, Rod M. The Market Potential of Grape Waste Alternatives. J Food Res. 2014;3(2):91.

McClean KH, Winson MK, Fish L, Taylor A, Chhabra SR, Camara M, et al. Quorum sensing and Chromobacterium violaceum: Exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones. Microbiology. 1997;143(12):3703-11.

Cao H, Lai Y, Bougouffa S, Xu Z, Yan A. Comparative genome and transcriptome analysis reveals distinctive surface characteristics and unique physiological potentials of Pseudomonas aeruginosa ATCC 27853. BMC Genomics. 2017;18(1).

Harshey RM, Matsuyama T. Dimorphic transition in Escherichia coli and Salmonella typhimurium: Surface-induced differentiation into hyperflagellate swarmer cells. Proc Natl Acad Sci U S A. 1994;91(18):8631-5.

Rai N, Rai R, Venkatesh K V. Quorum Sensing vs Quorum Quenching: A Battle with No End in Sight. Kalia VC, editor. New Delhi: Springer India. 2015:173-183.

Chu W, Vattem DA, Maitin V, Barnes MB, McLean RJC. Bioassays of quorum sensing compounds using Agrobacterium tumefaciens and Chromobacterium violaceum. Methods Mol Biol. 2011;692:3-19.

Husain FM, Ahmad I, Al-Thubiani AS, Abulreesh HH, AlHazza IM, Aqil F. Leaf extracts of Mangifera indica L. inhibit quorum sensing - Regulated production of virulence factors and biofilm in test bacteria. Front Microbiol. 2017;8.

Sybiya Vasantha Packiavathy IA, Agilandeswari P, Musthafa KS, Karutha Pandian S, Veera Ravi A. Antibiofilm and quorum sensing inhibitory potential of Cuminum cyminum and its secondary metabolite methyl eugenol against Gram negative bacterial pathogens. Food Res Int. 2012;45(1):85-92.

Di Donato P, Taurisano V, Tommonaro G, Pasquale V, Jiménez JMS, de Pascual-Teresa S, et al. Biological Properties of Polyphenols Extracts from Agro Industry’s Wastes. Waste and Biomass Valorization. 2018;9(9):1567-78.

Kordbacheh H, Eftekhar F, pathogenesis SE-M, 2017 undefined. Anti-quorum sensing activity of Pistacia atlantica against Pseudomonas aeruginosa PAO1 and identification of its bioactive compounds. Microbial pathogenesis. 2017;110:390-398.

Niu K, Kuk M, Jung H, Chan K, Kim S. Leaf Extracts of Selected Gardening Trees Can Attenuate Quorum Sensing and Pathogenicity of Pseudomonas aeruginosa PAO1. Indian J Microbiol. 2017;57(3):329-38.

De Marco S, Piccioni M, Pagiotti R, Pietrella D. Antibiofilm and Antioxidant Activity of Propolis and Bud Poplar Resins versus Pseudomonas aeruginosa. Evidence-based Complement Altern Med. 2017;2017.

Published

2020-06-26