Inhibición de motilidad y Quorum sensing bacteriano por desechos de vinificación

  • MARIO ARENA INBIOFAL

Abstract

In the food industry, biofilm is the main cause of contamination. Due to food regulations, only a few compounds can be used as preservatives or be in contact with food during their preparation and at the same time, they must be economical. An alternative is the use of by-products or agro-industry waste. One of the main food industry in Latin America is viticulture. The present investigation uses marc and stems of Torrontés varietal wine, to inhibit the bacterial virulence controlled by Quorum sensing (QS) and mainly its motility (swarming and swimming), which would allow the displacement and formation of biofilm in foods. We perform extractions with solvents of increasing polarity (hexane, chloroform, ethyl acetate, and methanol). We challenge 250 and 500 µg / ml of extracts to the strains of Pseudomonas aeruginosa ATCC 27853 and LVP65. Methanolic extracts to marc and stems inhibited more than 50 swarming motility of both strains. In addition, both ethyl acetate extracts inhibited the swimming (25-30%). The anti-QS activity of the Extracts was tested with strains Chromobacterium violaceum ATCC 12472 and CV026. Determining an inhibition of the methanolic and acetate extracts on the QS system. The bioactivities found to make these wastes a novel alternative for food preservation

Published
2020-06-26