Study of the in vitro and in vivo bioactivity of glucosinolates/isothiocyanates-rich broccoli sprouts
Broccoli sprouts are very interesting plant foods due to their potential source of nutrients and bioactive compounds. These sprouts have a higher content in glucosinolates than adult plants, being glucoraphanin the one presented in higher amount. Its hydrolysis product, the isothiocyanate sulforaphane, has shown different biological activities in previous studies, such as antiinflamatory, antioxidant and antiproliferative effect. The aim of this work was to explore the antiproliferative effect of pure glucoraphanin and pure sulforaphane against cell lines of human cancer in vitro, compared to the complete broccoli sprouts. Results showed that the whole plant is more active. On the other hand, also the antinociceptive effect of broccoli sprouts was studied in rodent models in vivo, providing an interesting research line for the study of the action pathways of these natural products as phytotherapeutics.
Keywords: crucifers, glucoraphanin, sulforaphane, antiproliferative, antinociceptive.